尿激酶型纖溶酶原激活因子受體(uPAR)重組蛋白

Recombinant Plasminogen Activator, Urokinase Receptor (uPAR)

CD87; PLAUR; PLAU-R; U-PAR; URKR; Monocyte Activation Antigen Mo3

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Plasminogen Activator, Urokinase Receptor

Synonyms: Synonyms: Synonyms: Synonyms: U-PAR, uPAR

Species: Species: Species: Species: Rat

Size: 100 μg

Source: Source: Source: Source: Escherichia coli-derived

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Isoform 1: cell membrane; Lipid-anchor; GPI-anchor; Isoform

2:secreted.

[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

Residues Residues Residues Residues: Thr17~Asn212 (Accession # P49616), with one N-terminal His Tag.

Grade & Purity: Purity: Purity: Purity: >95%, 25 kDa as determined by SDS-PAGE reducing conditions.

Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in Phosphate buffered saline(PBS), pH 7.4，

containing 0.01% sarcosyl, 5% sucrose. Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 23.2 kDa

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 6.3

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in PBS, PH7.2-PH7.4 .

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Stability: Stability: Stability: Stability: The stability of protein is determined by the loss rate of concentration. The loss rate was determined by

accelerated thermal degradation test.The loss of this protein is less than 5% within the expiration date under

appropriate storage condition.

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months .

[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES]

The target protein is fused with one N-terminal His tag.

TCVP ASQGLRCIQC ESNQDCLVEE CALGQDLCRT TVLREWEDAE ELEVVTRGCA HKEKTNRTMSYRMGSVIVSL TETVCATNLC NRPRPGARGR PFPRGRYLEC ASCTSLDQSC ERGREQSLQC RYPTEHCIEVVTLQSTERSV KDEPYTKGCG SLPGCPGTAG FHSNQTFHFL KCCNFTQCNG GPVLDLQSLP PN

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1. Rabbani S A., et.al. (1994) FEBS Lett. 338:69-74.

?2. The MGC Project Team (2004) Genome Res. 14:2121-2127.

3. Ragno P., et.al. (1992) FEBS Lett. 306:193-198.

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