F-框蛋白32(FBXO32)重組蛋白
Recombinant F-Box Protein 32 (FBXO32)
ATROGIN1; Fbx32; MAFbx; Muscle atrophy F-box protein
[ PROPERTIES ]
Source: Prokaryotic expression.
Host: E. coli
Residues: Met1~Glu201
Tags: N-terminal His-Tag
Tissue Specificity: Cardiac Muscle, Skeletal Muscle.
Subcellular Location: Cytoplasm, nucleus. Purity: >98%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA,
1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Original Concentration: 200μg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 9.6
Predicted Molecular Mass: 27.2kDa
Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
?[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.
[ SEQUENCE ]
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