N-甲基嘌呤DNA糖基化酶(MPG)重組蛋白

Recombinant N-Methylpurine DNA Glycosylase (MPG)

AAG; APNG; ADPG; CRA36.1; MDG; Mid1; PIG11; PIG16; Anpg; DNA-3-Methyladenine Glycosylase; Alkyladenine DNA Glycosylase; 3-alkyladenine DNA glycosylase

[ PROPERTIES ]

Residues: Pro70~Lys220 and Leu221~Thr296 linked

by a peptide LCQALA

Tags: N-terminal His-Tag

Accession: P29372

Host: E. coli

Subcellular Location: Cytoplasm. Mitochondrion

matrix, mitochondrion nucleoid. Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 9.6

Predicted Molecular Mass: 34.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

P YRSIYFSSPK GHLTRLGLEF FDQPAVPLAR AFLGQVLVRR LPNGTELRGR IVETEAYLGPEDEAAHSRGG RQTPRNRGMF MKPGTLYVYI IYGMYFCMNI SSQGDGACVL LRALEPLEGLETMRQLRSTL RKGTASRVLK DRELCSGPSK LCQALA LCQALAINKS FDQRDLAQDEAVWLERGPLE PSEPAVVAAA RVGVGHAGEW ARKPLRFYVR GSPWVSVVDR VAEQDT

[ REFERENCES ]

1. Samson L., et al. (1991) Proc. Natl. Acad. Sci. U.S.A. 88:9127-9131.

2. Vickers M.A., et al. (1993) Proc. Natl. Acad. Sci. U.S.A. 90:3437-3441.

3. Chakravarti D., et al. (1991) J. Biol. Chem. 266:15710-15715.

4. O'Connor T.R., Laval J. (1991) Biochem. Biophys. Res. Commun. 176:1170-1177.

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