細胞色素P450家族成員11A1(CYP11A1)重組蛋白

Recombinant Cytochrome P450 11A1 (CYP11A1)

CYP11A; P450SCC; Cholesterol desmolase; Cholesterol Side Chain Cleavage Enzyme; Cytochrome P450 Family 11 Subfamily A Polypeptide 1

[ PROPERTIES ]

Residues: Val391~Gln515

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P00189

Host: E. coli

Subcellular Location: Mitochondrion membrane.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.3

Predicted Molecular Mass: 17.4kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

VTLQRYPESD LVLQDYLIPA KTLVQVAIYA MGRDPAFFSS PDKFDPTRWL SKDKDLIHFRNLGFGWGVRQ CVGRRIAELE MTLFLIHILE NFKVEMQHIG DVDTIFNLIL TPDKPIFLVFRPFNQ

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