雙絲氨酸/蘇氨酸酪氨酸蛋白激酶(DSTYK)重組蛋白

Recombinant Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK)

RIPK5; Dusty PK; RIP5; SgK496; Receptor Interacting Protein Kinase 5; RIP-homologous kinase; Sugen kinase 496; Receptor-interacting serine/threonine-protein kinase 5

[ PROPERTIES ]

Residues: Pro650~Leu904

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q6XUX2

Host: E. coli

Subcellular Location: Cytoplasm. Cell membrane.

Apical cell membrane. Basolateral cell membrane.

Cell junction.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.6

Predicted Molecular Mass: 32.6kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

P KLGQELGRGQ YGVVYLCDNW GGHFPCALKS VVPPDEKHWN DLALEFHYMRSLPKHERLVD LHGSVIDYNY GGGSSVAVLL IMERLHRDLY TGLKAGLSLE TRLQIALDVVEGIRFLHSQG LVHRDIKLKN VLLDKQNRAK ITDLGFCKPEAMMSGSIVGT PIHMAPELFTGKYDNSVDVY AFGILFWYIC SGSIKLPEAFERCASKDHLW NNVRRGTRPE RLPVFDEECWQLMEACWDGD PSKRPLLGIV QPIL

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