Ig樣域蛋白粘附分子1(AMIGO1)重組蛋白

Recombinant Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1)

ALI2; Alivin-2; Amphoterin-Induced Gene And Open Reading Frame; Amphoterin-induced protein 1

[ PRODUCT INFORMATION ]

Immunogen: AMIGO1, Human

Clonality: Polyclonal

Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200μg/mL

UOM: 100μg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant AMIGO1 (Gly28~Thr372) with two N-terminal Tags,

His-tag and T7-tag expressed in E.coli.

Accession No.: RPQ889Hu01

[ ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against AMIGO1. It has been

selected for its ability to recognize AMIGO1 in immunohistochemical staining and

western blotting.

[ APPLICATIONS ]

Western blotting: 1:50-400

Immunocytochemistry in formalin fixed cells: 1:50-500

Immunohistochemistry in formalin fixed frozen section: 1:50-500

Immunohistochemistry in paraffin section: 1:10-100

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant AMIGO1 (Gly28~Thr372)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,

BPB 0.01%, NaN3 0.02%.

[ STORAGE ]

Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezerfor one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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