3-羥基3-甲基戊二酰輔酶A還原酶(HMGCR)重組蛋白
Recombinant 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR)
HMG-CoA Reductase; Hydroxymethylglutaryl-CoA Reductase
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Ala638~Ala887
Tags: N-terminal His-Tag
Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane
protein. Purity: >99%
Traits: Freeze-dried powder
Buffer formulation: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM
EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200μg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 8.4
Predicted Molecular Mass: 30.0kDa
Accurate Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of0.1-1.0 mg/mL. Do not vortex.
?[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months.?
Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date under
appropriate storage condition.
[ SEQUENCE ]
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