氧鯊烯環(huán)化酶(OSC)重組蛋白

Recombinant Oxidosqualene Cyclase (OSC)

LSS; Lanosterol Synthase; 2,3-epoxysqualene--lanosterol cyclase; Oxidosqualene--lanosterol cyclase

[ DESCRIPTION ]

Protein Names: Oxidosqualene Cyclase

Synonyms: OSC, LSS

Species: Human

Size: 100μg

Source: Escherichia coli-derived

Subcellular Location: Endoplasmic reticulum

membrane; Peripheral membrane protein.

[ PROPERTIES ]

Residues: Asp388~Pro549 (Accession # P48449),

with N-terminal His-Tag.

Grade & Purity: >95%, 22kDa as determined by

SDS-PAGE reducing conditions.

Formulation: Supplied as lyophilized form in PBS, pH

7.4, containing 5% sucrose, 0.01% sarcosyl.

Endotoxin Level: <1.0 EU per 1μg (determined by

the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 19.9kDa

Predicted isoelectric point: 6.3

Human OSC?

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

?Store at 2-8oC for one month.

?Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF-DTA FAIQALLEAG GHHRPEFSSC LQKAHEFLRL SQVPDNPPDYQKYYRQMRKG GFSFSTLDCG WIVSDCTAEA LKAVLLLQEK CPHVTEHIPR ERLCDAVAVLLNMRNPDGGF ATYETKRGGH LLELLNPSEV FGDIMIDYTY VECTSAVMQA LKYFHKRFP?

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