角蛋白14(CK14)重組蛋白

Recombinant Cytokeratin 14 (CK14)

KRT14; EBS3; EBS4; K14; Keratin 14; Epidermolysis Bullosa Simplex Dowling-Meara Koebner; Keratin, type I cytoskeletal 14

[ PROPERTIES ]

Residues: Glu122~Glu429 (Accession # Q6IFV1),

with two N-terminal Tags, His-tag and T7-tag. Host: E. coli

Subcellular Location: Cytoplasm. Nucleus.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.0

Predicted Molecular Mass: 39.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- EKVTMQNLN DRLATYLDKVRALEEANSDL EVKIRDWYQR QRPTEIKDYS PYFKTIEDLK SKILAATVDN ANVLLQIDNARLAADDFRTK FETEQSLRIN VESDINGLRR VLDELTLARA DLEMQIESLK EELAYLKKNHEEEMASMRG Q VGGDV NVEMD AAPGV D LS RI LNEMRDQYEK MAEKNRK DAEDWFFTKTEEL NREVATNSEL VQSGKSEISE LRRTMQNLEI ELQSQLSMKA SLENNLEETKGRYCMQLAQI QEMIGSVEEQ LAQLRCEMEQ QNQEYKILLD VKTRLEQEIA TYRRLLEGE

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