晝夜自發(fā)輸出周期蛋白kaput(CLOCK)重組蛋白

Recombinant Circadian Locomoter Output Cycles Protein Kaput (CLOCK)

KAT13D; bHLHe8; Class E basic helix-loop-helix protein 8

[ PROPERTIES ]

Residues: Ala34~Ala379

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: O15516

Host: E. coli

Subcellular Location: Nucleus. Cytoplasm.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.2

Predicted Molecular Mass: 44.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

AKRVSRN KSEKKRRDQF NVLIKELGSM LPGNARKMDK STVLQKSIDF LRKHKEITAQSDASEIRQDW KPTFLSNEEF TQLMLEALDG FFLAIMTDGS IIYVSESVTS LLEHLPSDLVDQSIFNFIPE GEHSEVYKIL STHLLESDSL TPEYLKSKNQ LEFCCHMLRG TIDPKEPSTYEYVKFIGNFK SLNSVSSSAH NGFEGTIQRT HRPSYEDRVC FVATVRLATP QFIKEMCTVEEPNEEFTSRH SLEWKFLFLD HRAPPIIGYL PFEVLGTSGY DYYHVDDLEN LAKCHEHLMQ

YGKGKSCYYR FLTKGQQWIW LQTHYYITYH QWNSRPEFIV CTHTVVSYA

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