Y-框結(jié)合蛋白1(YBX1)重組蛋白

Recombinant Y-Box Binding Protein 1 (YBX1)

BP8; CSDA2; CSDB; DBPB; MDR-NF1; NSEP1; YB1; Nuclease Sensitive Element Binding Protein 1; CCAAT-binding transcription factor I subunit A; DNA-binding protein B

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Gln134~Glu324

Tags: N-terminal His-Tag

Tissue Specificity: Liver, Brain. Subcellular Location: Cytoplasm. Nucleus. Cytoplasmic granule. Purity: >95%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200μg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 10.3

Predicted Molecular Mass: 26.2kDa

Accurate Molecular Mass: 26&36kDa as determined by SDS-PAGE reducing

conditions.?

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.?

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for two years.

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date under

appropriate storage condition.

[ SEQUENCE ]

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