膜聯(lián)蛋白A11(ANXA11)重組蛋白

Recombinant Annexin A11 (ANXA11)

ANX-A11; ANX11; CAP50; 56 kDa autoantigen; Calcyclin-associated annexin 50

[ PROPERTIES ]

Residues: Met1~Asp505

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P50995

Host: E. coli

Subcellular Location: Cytoplasm, Melanosome,

Nucleus envelope, Nucleus, nucleoplasm,

cytoskeleton, spindle.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 7.5

Predicted Molecular Mass: 58.1kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MSY PGY PP PP GGY PPA AP G G GPWG GAAY PP PPSMP PIG L D NVAT YAG Q F NQDYLSGMAAN MSGTFGGANM PNLYPGAPGA GYPPVPPGGF GQPPSAQQPVPPYGMYPPPG GNPPS RMPSY PPYPGAPVPG QPMPPPGQQP PGAYPGQPPVTYPGQPPVPL PGQQQPVPSY PGYPGSGTVT PAVPPTQFGS RGTITDAPGF DPLRDAEVLRKAMKGFGTDE QAIIDCLGSR SNKQRQQILL SFKTAYGKDL IKDLKSELSG NFEKTILALMKTPVLFDIYE IKEAIKGVGT DEACLIEILA SRSNEHIREL NRAYKAEFKK TLEEAIRSDTSGHFQRLLIS LSQGNRDEST NVDMSLAQRD AQELYAAGEN RLGTDESKFN AVLCSRSRAHLVAVFNEYQR MTGRDIEKSI CREMSGDLEE GMLAVVKCLK NTPAFFAERL NKAMRGAGTKDRTLIRIMVS RSETDLLDIR SEYKRMYGKS LYHDISGDTS GDYRKILLKI CGGND

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