載脂蛋白A1(APOA1)重組蛋白
Recombinant Apolipoprotein A1 (APOA1)
Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Asp25~Gln264
Tags: N-terminal His-Tag
Tissue Specificity: Protein of plasma HDL, Chylomicrons. Subcellular Location: Secreted. Purity: >92%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200μg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.3
Predicted Molecular Mass: 31.6kDa
Accurate Molecular Mass: 28kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as
follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc.?
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate the
protein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date under
appropriate storage condition.?
[ SEQUENCE ]
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