含CASP2和RIPK1域死亡域銜接蛋白(CRADD)重組蛋白

Recombinant CASP2 And RIPK1 Domain Containing Adaptor With Death Domain Protein (CRADD)

RAIDD; RIP-Associated ICH1/CED3-Homologous Protein With Death Domain; Caspase and RIP adapter with death domain; Death domain-containing protein CRADD

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: CASP2 And RIPK1 Domain Containing Adaptor With Death Domain

Protein

Gene Names: CRADD

Size: 100μg

Source: Recombinant

Expression Expression Expression Expression Host: E.coli

Function: Function: Function: Function: Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF

receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits

caspase-2 to the TNFR-1 signalling complex.

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm. Nucleus.

Tissue Specificity: Specificity: Specificity: Specificity: Constitutively expressed in most tissues, with particularly high

expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.

[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

Residues Residues Residues Residues: Met1~Glu199 (Accession # P78560), with a N-terminal His-tag.

Grade & Purity: Purity: Purity: Purity: >95%, 24 kDa as determined by SDS-PAGE reducing conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg(determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA;IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 24.26 kDa

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in PBS.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term

storage. Avoid repeated freeze/thaw cycles.

Valid period: period: period: period: 12 months stored at -80oC.

[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]

The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will

increase the chelating ability of the tag to Ni-Sepharose during purification.

MGHHHHHHSGSEF-MEARDKQVLR SLRLELGAEV LVEGLVLQYL YQEGILTENH IQEINAQTTG LRKTMLLLDILPSRGPKAFD TFLDSLQEFP WVREKLKKAR EEAMTDLPAG DRLTGIPSHI LNSSPSDRQI NQLAQRLGPEWEPMVLSLGL SQTDIYRCKA NHPHNVQSQV VEAFIRWRQR FGKQATFQSL HNGLRAVEVD PSLLLHMLE

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1. Chou J.J., et al. (1998) Cell 94:171-180.?

2. Ahmad M., et al. (1997) Cancer Res 57 (4): 615–619.

3. Vakifahmetoglu H., et al. (2006) Oncogene 25:5683-5692.?

4. Duan H., et al. (1997) Nature 385:86-89. 5. The MGC Project Team (2004) Genome Res. 14:2121-2127.

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