陰離子/糖轉(zhuǎn)運(yùn)蛋白(AST)重組蛋白

Recombinant Anion/Sugar Transporter (AST)

SLC17A5; SD; ISSD; NSD; SIALIN; SIASD; SLD; Solute Carrier Family 17 Member 5; Sialic Acid Storage Disease; Vesicular H(+)/Aspartate-glutamate cotransporter

[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]

Residues: Residues: Residues: Residues: Thr146~Phe405 (Accession # Q9NRA2),

with two N-terminal Tags, His-tag and T7-tag. Host: E. coli

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cell membrane;

Multi-pass membrane protein. Cytoplasmic

vesicle, secretory vesicle, synaptic vesicle

membrane. Lysosome membrane.

Purity: Purity: Purity: Purity: >95%

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 9.2

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 33.0kDa

Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF -TAVLT LFTPIAAD LGVGPLIVLRAL EGLGEGVTFP AMHAMWSSWA PPLERSKLLS ISYAGAQLGT VISLPLSGIICYYMNWTYVF YFFGTIGIFW FLLWIWLVSD TPQKHKRISH YEKEYILSSL RNQLSSQKSVPWVPILKSLP LWAIVVAHFS YNWTFYTLLT LLPTYMKEIL RFNVQENGFL SSLPYLGSWLCMILSGQAAD NLRAKWNFST LCVRRIFSLI GMIGPAVFLV AAGFIGCDYS LAVAFLTISTTLGGF

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