含KH域RNA結(jié)合信號通路關(guān)聯(lián)蛋白1(KHDRBS1)重組蛋白

Recombinant KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1)

Sam68; p62; GAP-Associated Tyrosine Phosphoprotein p62; Src-associated in mitosis 68 kDa protein; p21 Ras GTPase-activating protein-associated p62

[ PROPERTIES ]

Residues: Leu156~Glu381 (Accession # Q60749),

with two N-terminal Tags, His-tag and T7-tag. Host: E. coli

Subcellular Location: Nucleus. Membrane.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 9.5

Predicted Molecular Mass: 28.2kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSS HHHHHH SSG LVP RGS H MASMTGGQQM GRGS - LKE RV LIPVKQYPKFNFVGKILGPQ GNTIKRLQEE TGAKISVLGK GSMRDKAKEE ELRKGGDPKY AHLNMDLHVFIEVFGPPCEA YALMAHAMEE VKKFLVPDMM DDICQEQFLE LSYLNGVPEP SRGRGVSVRGRGAAPPPPPV PRGRGVGPPR GALVRGTPVR GSITRGATVT RGVPPPPTVR GAPTPRARTAGIQRIPLPPT PAPETYEDYG YDDTYAEQSY E

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