含CASP8和FADD樣凋亡調(diào)節(jié)因子(CFLAR)重組蛋白

Recombinant CASP8 And FADD Like Apoptosis Regulator (CFLAR)

CASH; CASP8AP1; CLARP; Casper; FLAME1; FLIP; I-FLICE; MRIT; USURPIN; c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; Caspase-eight-related protein; FADD-like antiapoptotic molecule 1

[ PROPERTIES ]

Residues: Arg43~Tyr281

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: C0H5Y5

Host: E. coli

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 8.9

Predicted Molecular Mass: 31.2kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

RDLLDCLS ERGQLSFAAL AELLYRVRRF DLLKRILKTD KAAVEDHLCR SPHLVSDYRVLLMEIGENLN QSDVSSLIFL TKDYTGRGKV AKDKSFLDLV IELEKLNLIG SDQLNLLEKCLKSIHRIDLK TKIQKYTQSS QEARSNMNAL QASLPKLSIK EHLYNSRLQN GRSKEPRFVEHHGIQRKPVK TSIQESGIFL PPHIHEESYR MQSKPLGICL IIDCIGNDTE YLRETFTSLG Y

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