毒蕈堿型膽堿受體M1(CHRM1)重組蛋白

Recombinant Cholinergic Receptor, Muscarinic 1 (CHRM1)

CHR-M1; HM1; M1; M-AChRM1; Muscarinic Acetylcholine Receptor M1

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Cholinergic Receptor, Muscarinic 1

Synonyms: Synonyms: Synonyms: Synonyms: CHRM1, Chrm-1

Species: Species: Species: Species: Rat

Size: 100μg

Source: Source: Source: Source: Escherichia coli-derived

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cell membrane; Multi-pass

membrane protein. Cell junction, synapse,

postsynaptic cell membrane.

[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]

Residues: Residues: Residues: Residues: Arg210~Ala363 (Accession # P08482),

with N-terminal His-Tag. Grade & Purity: Purity: Purity: Purity: >95%, 24kDa as determined by

SDS-PAGE reducing conditions.

Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS, pH

7.4, containing 0.1% Sarcosyl, 5% sucrose.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by

the LAL method).

Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 18.3kDa

Rat CHRM1 kDa

9466.24533262014.4

15% SDS-PAGE SDS-PAGE SDS-PAGE SDS-PAGE

SDS-PAGE is a technique that separates proteins based on size. However, the actual band size

observed may differ from the predicted; the common factors may include three terms:

1. Alternative splicing (splice variants).

2. 2. The composition of amino acids may give the protein the different relative charge.

3. Polymerization of the target protein.

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 9.1

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF-R IYRETENRAR ELAALQGSET PGKGGGSSSS SERSQPGAEGSPESPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV

VIKMPMVDSE AQAPTKQPPK SSPNTVKRPT KKGRDRGGKG QKPRGKEQLAKRKTFSLVKE KKA

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1. Takamori M., et al. (2007) Eur. J. Neurol. 14:1230-1235.

2. Maeda Y., et al. (2006) Am. J. Respir. Crit. Care Med. 174:1119-1124.

3. Santafe M.M., et al. (2003) Eur. J. Neurosci. 17:119-127.

4. Anagnostaras S.G., et al. (2003) Nat. Neurosci. 6:51-58.

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