碘甲腺原氨酸脫碘酶Ⅱ(DIO2)重組蛋白

Recombinant Deiodinase, Iodothyronine, Type II (DIO2)

5DII; D2; SelY; TXDI2; Thyroxine Deiodinase,Type II; Type-II 5'-deiodinase

[ PROPERTIES ]

Residues: Ser30~Phe259 (Accession # Q9Z1Y9),

with two N-terminal Tags, His-tag and MBP-tag.

Host: E. coli

Subcellular Location: Membrane; Single-pass

membrane protein . Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.8

Predicted Molecular Mass: 72.4kDa

Accurate Molecular Mass: 60kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and MBP-tag, itssequence is listed below.

MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDIIFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNKDLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIKDVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSKVNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL

GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDEALKDAQTGST SGSGHHHHHH SAGLVPRGST AIGMKETAAA KFERQHMDSP DLGTLEVLFQGPLGSEF- S VILLKHVALL LSRSKSTRGE WRRMLTSEGL RCVWNSFLLD AYKQVKLGEDAPNSSVVHVS NPESGNNYAS EKTADGAECH LLDFASAERP LVVNFGSATU PPFTRQLPAFRQLVEEFSSV ADFLLVYIDE AHPSDGWAVP GDSSLSFEVK KHRNQEDRCA AAHQLLERFS

LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG KGPFSYNLQE VRSWLEKNF

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