脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)重組蛋白

Recombinant Phospholipase A2 Group VII (LpPLA2)

PLA2G7; PAF-AH; PAFAH; Lp-PLA2; LDL-PLA2; Platelet Activating Factor Acetylhydrolase,Plasma; Phospholipase A2,Group VII; LDL-associated phospholipase A2; Phospholipase A2, Lipoprotein Associated

[ PROPERTIES ]

Residues: Ile22~Asp444 (Accession # Q28262),

with N-terminal His-Tag.

Host: E. coli

Subcellular Location: Secreted, extracellular

space.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 6.1

Predicted Molecular Mass: 50.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSG SEFELRRQ- IDWQDLNPV AHIRSSAWAN KIQALMAAAS IRQSRIPKGNGSYSVGCTDL MFDYTNKGTF LRLYYPSQED DHSDTLWIPN KEYFFGLSKY LGTPWLMGKILSFFFGSVTT PANWNSPLRT GEKYPLIVFS HGLGAFRTIY SAIGIDLASH GFIVAAIEHRDGSASATYYF KDQSAAEIGN KSWSYLQELK PGDEEIHVRN EQVQKRAKEC SQALNLILDIDHGRPIKNVL DLEFDVEQLK DSIDRDKIAV IGHSFGGATV LQALSEDQRF RCGIALDAWMLPLDDAIYSR IPQPLFFINS ERFQFPENIK KMKKCYSPDK ERKMITIRGS VHQNFADFTFTTGKIVGYIF TLKGDIDSNV AIDLCNKASL AFLQKHLGLR KDFDQWDSLI EGKDENLMPG

TNINITNEHD TLQNSPEAEK SNLD

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