肘臀蛋白(CUBN)重組蛋白

Recombinant Cubilin (CUBN)

IFCR; MGA1; gp280; Intrinsic Factor Cobalamin Receptor; 460 kDa receptor; Intestinal intrinsic factor receptor; Intrinsic factor-vitamin B12 receptor

[ DESCRIPTION ]

Protein Names: Cubilin

Synonyms: CUBN, Ifcr

Species: Rat

Size: 100μg

Source: Escherichia coli-derived

Subcellular Location: Endosome membrane; Peripheral membrane protein. Lysosome

membrane.

[ PROPERTIES ]

Residues: Cys3157~Val3274 (Accession # O70244), with N-terminal His-Tag.

Grade & Purity: >95%, 14 kDa as determined by SDS-PAGE reducing conditions.

Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.

?Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 14.4 kDa

Predicted isoelectric point: 5.5

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.?

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

?Store at 2-8oC for one month.

?Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate wasdetermined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obviousdegradation and precipitation were observed. (Referring from China Biological Products Standard, which wascalculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under

appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF-CGGY LTEDNQSFVS PDSDSNGRYD KGLSCIWYIV APENKLVKLT FNVFTLEGPS SAGSCVYDYVQIADGASINS YLGGKFCGSR MPAPFISSGN FLTFQFVSDV TVEMRGFNAT YTFV?

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