微粒體甘油三酸酯轉(zhuǎn)運(yùn)蛋白(MTTP)重組蛋白

Recombinant Microsomal Triglyceride Transfer Protein (MTTP)

MTP; ABL; Microsomal triglyceride transfer protein large subunit

[ PROPERTIES ]

Residues: Leu28~Gly459

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: O08601

Host: E. coli

Subcellular Location: Endoplasmic reticulum.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 8.6

Predicted Molecular Mass: 47.1kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

LNN ERLYKLTYST EVFLDGGKGK PQDSVGYKIS SDVDVVLLWR NPDGDDDQVIQVTITAVNVE NAGQQRGEKS IFQGKSTPKI IGKDNLEALQ RPMLLHLVRG KVKEFYSYENEPVGIENLKR GLASLFQMQL SSGTTNEVDI SGDCKVTYQA QQDKVVKIKA LDTCKIERSGFTTANQVLGV SSKATSVTTY KIEDSFVTAV LAEETRAFAL NFQQTIAGKI VSKQKLELKTTEAGPRMIPG KQVAGVIKAV DSKYKAIPIV GQVLERVCKG CPSLAEHWKS IRKNLEPENLSKAEAVQSFL AFIQHLRTSR REEILQILKA EKKEVLPQLV DAVTSAQTPD SLEAILDFLDFKSDSSIILQ ERFLYACGFA THPDEELLRA LLSKFKGSFA SNDIRESVMI IIGALVRKLCQNEGCKLKAV VEAKKLILG

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