鈉氫交換調(diào)控因子2(SLC9A3R2)重組蛋白

Recombinant Sodium Hydrogen Exchange Regulatory Cofactor 2 (SLC9A3R2)

TKA1; SIP1; E3KARP; NHERF2; OCTS2; SRY-interacting protein 1; Solute Carrier Family 9 Member 3 Regulator 2; Tyrosine Kinase Activator Protein 1

[ PROPERTIES ]

Residues: Leu56~Phe337

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q15599

Host: E. coli

Subcellular Location: Endomembrane system;

Peripheral membrane protein. Nucleus. Apical

cell membrane.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 6.7

Predicted Molecular Mass: 35.2kDa

Accurate Molecular Mass: 44kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

?Store at 2-8

oC for one month.

?Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

LVEVN GVNVEGETHH QVVQRIKAVE GQTRLLVVDQ ETDEELRRRQ LTCTEEMAQRGLPPAHDPW E PKPDWAHTGS HSSEAGKKDV SGPLRELRPR LCHLRKGPQGYGFNLHSDKS RPGQYIRSVD PGSPAARSGL RAQDRLIEVN GQNVEGLRHA EVVASIKAREDEARLLVVDP ETDEHFKRLR VTPTEEHVEG PLPSPVTNGT SPAQLNGGSA CSSRSDLPGSDKDTEDGSAW KQDPFQESGL HLSPTAAEAK EKARAMRVNK RAPQMDWNRK REIFSNF

[ REFERENCES ]

1. Poulat F., et al. (1997) J. Biol. Chem. 272:7167-7172.

2. Reczek D., et al. (1997) J. Cell Biol. 139:169-179.

3. Yun C.H.C., et al. (1997) Proc. Natl. Acad. Sci. U.S.A. 94:3010-3015.

4. Imai K., et al. (1998) Gene 222:287-295.

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